Abstract
5-Methyl-5,6,7,8-tetrahydrofolic (5-methylTHF) acid can be measured in human serum and whole blood by fluorescence after deproteinization with perchloric acid and separation by high-performance liquid chromatography (HPLC). The technique is sensitive, rapid, and correlates with existing methods for total folates.
MeSH terms
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Chromatography, High Pressure Liquid / methods
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Humans
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Indicators and Reagents
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Lacticaseibacillus casei / drug effects
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Lacticaseibacillus casei / growth & development
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Spectrometry, Fluorescence
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Tetrahydrofolates / blood*
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Tetrahydrofolates / pharmacology
Substances
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Indicators and Reagents
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Tetrahydrofolates
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5-methyltetrahydrofolate